December 2019
Looked for teammates to set up iGEM SZPT-CHINA team in 2020
January 2020
Team members brainstorm
February 2020
The goal of the project was to prevent and diagnose dental caries
March 2020
Investigated the current situation of dental caries and the existing treatment methods on the market, found and read related articles
April 2020
Consulted the data and determined the experimental direction
May 2020
Consulted the data and made clear the general practical route
June 1-15, 2020
Understood and designed the experimental scheme, and participated in the South China exchange meeting
All of the team members received the laboratory safety training, including understanding the importance of laboratory safety, using and protecting hazardous chemicals, understanding laboratory hazards, doing experiments in laboratory under safe conditions to establish personal safety awareness
June 16-30, 2020
Laboratory skill training was carried out
July 2020
The recombinant expression vector pET28a (+) - pelB-comD-comE-nlmC-sfGFP was successfully constructed
The relationship between the detection system and CSP were completed that proves the detection system is effective and feasible
We had constructed the detection system of E.coli and Streptococcus mutans co-culture, but there was no significant difference in fluorescence intensity after 24 hours of co-culture. It was required to optimize the co-culture conditions
We found that nlmC had fluorescence leakage, so we replaced nlmC gene with nlmAB gene. Although the fluorescence leakage of nlmAB was lower than that of nlmC. We also found that the excitation fluorescence sensitivity of nlmAB was much lower than that of nlmC. Therefore, the nlmC with high sensitivity was selected.
We replaced the sfGFP gene with lacZ gene to make it hydrolyze X-gal to turn blue to achieve the purpose of visible color change by the naked eye to reduce the ultraviolet irradiation part of fluorescence excitation
August 2020
Nine antimicrobial peptides were screened.
The antibacterial peptide polymer was constructed in series.
The expression vector of antimicrobial peptide was successfully constructed.
The antibacterial peptide-protein was purified.
The enzyme hydrolysis and activity verification of the purified product of antimicrobial peptide showed that the antibacterial peptide monomer still had activity after purification and enzymatic hydrolysis. The antibacterial peptide polymer constructed by us can play its active role after purification and enzymatic hydrolysis into monomer
September 2020
The expression vector of antimicrobial peptide was successfully constructed.
The activity verification of antibacterial peptides secreted by lactic acid bacteria was completed, and the antibacterial peptides expressed by lactic acid bacteria could play an active role.
Added antimicrobial peptides to chewing gum
The plate validation of RalR was completed that proved that RalR could play a toxic role
The OD value of liquid culture was determined, and it was confirmed that RS was regulated by lysine
October 2020
Constantly improved project design and ideas
Completed all the experiments and HP.
Completed wiki, poster and upload.